Liposome-based flow-injection immunoassay system
نویسندگان
چکیده
منابع مشابه
Liposome-Based Flow Injection Immunoassay System
The development of many biochemical assays is dependent upon the specific interaction of an antigen with its antibody. This interaction is usually monitored using secondary labels with the most popular immunoassays employing fluorescent or radioactive tracers for detection of a single binding event. We are developing a novel flow injection analysis (FIA) system which contains an immunospecific ...
متن کاملLiposome-based flow-injection immunoassay for determining theophylline in serum.
We developed a method for quantitatively determining theophylline in serum, using a heterogeneous immunoassay called flow-injection immunoanalysis. The reaction involves competition between serum theophylline and theophylline-labeled liposomes. Separation occurs on a solid-phase reactor column containing immobilized antibody to theophylline incorporated in a flow-injection system. Subsequent ly...
متن کاملFlow Immunoassay Based on Sequential Injection Using Microbeads
A flow immunoassay based on a combined technique of the sequential injection with the beads injection using magnetic microbeads immobilized with antigen or antibody is described. A methodology of the present immunoassay based on chemiluminescence and electrochemical detection and its application to the determination of vitelloginin (Vg) and anionic surfactant, linear alkylbenzene sulfonate (LAS...
متن کاملNoncompetitive enzyme immunoassay for carcinoembryonic antigen by flow injection chemiluminescence.
BACKGROUND Recently, many automated immunoassay analyzers have been developed for carcinoembryonic antigen (CEA) to overcome the shortcomings in traditional immunoassay methods that are time-consuming and labor-intensive. Flow injection immunoassay (FIIA) has been increasingly applied to laboratory medicine due to its ease in automation, rapid speed and reproducible results. It is important to ...
متن کاملA membrane-based displacement flow immunoassay.
The use of a membrane-based continuous flow displacement immunoassay for detection of nanomolar quantities of explosives is demonstrated, and the kinetics of this system are characterized through experimentation. Antibodies of 2,4,6-trinitrotoluene (TNT) are immobilized onto a porous membrane with surface reactive sites designed to facilitate the covalent binding of the antibody. After saturati...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Research of the National Bureau of Standards
سال: 1988
ISSN: 0160-1741
DOI: 10.6028/jres.093.160